4DV5

Crystal structure of the Thermus thermophilus 30S ribosomal subunit with a 16S rRNA mutation, A914G, bound with streptomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.127, 402.127, 172.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.555 - 3.683
R-factor	0.1586
Rwork	0.156
R-free	0.21150
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.015
RMSD bond angle	1.821
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_978)
Refinement software	PHENIX (dev_978)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.830
High resolution limit [Å]	3.683	7.940	3.683
Rmerge	0.102	0.050	0.608
Number of reflections	149012
<I/σ(I)>	9.6
Completeness [%]	98.3	93.5	98.5
Redundancy	2.9	2.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	277	MPD, pH 6.5, HANGING DROP, temperature 277K