4DUK

Carboxypeptidase T with L-BENZYLSUCCINIC ACID

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8
Spacegroup name	P 63 2 2
Unit cell lengths	157.939, 157.939, 104.437
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.570
R-factor	0.15173
Rwork	0.151
R-free	0.16720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.125
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.570
Number of reflections	101808
Completeness [%]	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Counter diffusion		273	Counter diffusion, temperature 273K