4DTE
Crystal structure of zebrafish plasminogen activator inhibitor-1 (PAI-1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.160, 73.770, 91.750 |
Unit cell angles | 90.00, 102.73, 90.00 |
Refinement procedure
Resolution | 44.747 - 1.960 |
R-factor | 0.1751 |
Rwork | 0.174 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q02 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.757 |
Data reduction software | xia2 (II) |
Data scaling software | xia2 (II) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.150 | 2.130 | 2.010 |
High resolution limit [Å] | 1.960 | 2.080 | 1.960 |
Number of reflections | 60335 | ||
Completeness [%] | 98.8 | 99.4 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.1 | 277.15 | 20% (w/v) PEG3350, 0.2 M NaCl, 0.1 M bis-Tris, pH 6.1, VAPOR DIFFUSION, temperature 277.15K |