4DR2

Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with multiple copies of paromomycin molecules bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.596, 402.596, 177.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.389 - 3.249
R-factor	0.1731
Rwork	0.171
R-free	0.21090
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.032
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (dev_978)
Refinement software	PHENIX (dev_978)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.000	45.000	3.370
High resolution limit [Å]	3.249	6.990	3.249
Rmerge	0.115	0.041	0.811
Number of reflections	226409
<I/σ(I)>	7.8
Completeness [%]	99.6	97.2	100
Redundancy	3.7	3.6	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	277	MPD, pH 6.5, HANGING DROP, temperature 277K