4DQ2
Structure of staphylococcus aureus biotin protein ligase in complex with biotinol-5'-amp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 298 |
Collection date | 2009-09-09 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 94.598, 94.598, 130.626 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.550 - 2.500 |
R-factor | 0.19341 |
Rwork | 0.191 |
R-free | 0.23932 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 2.214 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.550 |
High resolution limit [Å] | 2.500 |
Number of reflections | 20793 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10% PEG 8000, 0.1M Tris-HCl pH 7.5, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |