4DPX
Crystal structure of S192A Staphylococcus epidermidis mevalonate diphosphate decarboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2011-06-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.922, 102.273, 155.942 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.591 - 1.948 |
R-factor | 0.164 |
Rwork | 0.162 |
R-free | 0.20010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qt5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.005 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.997 |
High resolution limit [Å] | 1.948 | 1.948 |
Rmerge | 0.083 | 0.382 |
Number of reflections | 46055 | |
<I/σ(I)> | 19.3 | 5.83 |
Completeness [%] | 98.6 | 94 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.25 M sodium formate, 16% w/v PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |