4DO0
Crystal Structure of human PHF8 in complex with Daminozide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 151.734, 151.734, 151.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 107.290 - 2.550 |
| R-factor | 0.21092 |
| Rwork | 0.208 |
| R-free | 0.26481 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wwu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.573 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.290 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.074 | 0.566 |
| Number of reflections | 19128 | |
| <I/σ(I)> | 24.4 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.5 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 277 | 0.1M acetate, 2M ammonium sulfate, pH 4.5, VAPOR DIFFUSION, temperature 277K |
