4DLQ

Crystal structure of the GAIN and HormR domains of CIRL 1/Latrophilin 1 (CL1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	65
Detector technology	CCD
Collection date	2009-07-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	124.000, 124.000, 77.305
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.900 - 1.850
R-factor	0.1646
Rwork	0.164
R-free	0.18540
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.092
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.900	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Number of reflections	58546
Completeness [%]	99.7	99.8	95.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	1.26M (NH4)2SO4, 0.1M sodium acetate pH 4.5, 0.2M NaCl,, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	1.26M (NH4)2SO4, 0.1M sodium acetate pH 4.5, 0.2M NaCl,, VAPOR DIFFUSION, HANGING DROP, temperature 293K