4DLA
Crystal structure of S-nitrosoglutathione reductase apoenzyme from tomato (Solanum lycopersicum)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-14 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 83.710, 125.000, 77.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.250 - 2.140 |
R-factor | 0.2401 |
Rwork | 0.237 |
R-free | 0.29710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1teh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.250 | 2.270 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.111 | 0.578 |
Number of reflections | 43940 | |
<I/σ(I)> | 9.21 | 2.59 |
Completeness [%] | 97.6 | 93.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 293 | 0.1 M MES pH 6.5, 12% PEG 20000, 4% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |