4DKX
Crystal Structure of the Rab 6A'(Q72L)
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-11-09 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 36.838, 96.780, 109.997 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.390 - 1.900 |
| R-factor | 0.19913 |
| Rwork | 0.196 |
| R-free | 0.25036 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 3.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.390 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 31899 | |
| Completeness [%] | 70.0 | 70 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 18% PEG 8000, 200mM Calcium acetate, 100mM Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
