4DKJ
CpG specific methyltransferase in complex with target DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-05 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 83.950, 83.950, 173.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.600 - 2.150 |
R-factor | 0.17832 |
Rwork | 0.176 |
R-free | 0.21631 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c7p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.253 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.078 | 0.310 |
Number of reflections | 34324 | |
<I/σ(I)> | 5.5 | 2.5 |
Completeness [%] | 99.3 | 99.9 |
Redundancy | 3.9 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 294 | 10% PEG 3350, 150 mM NaCl, 50 mM sodium. For cryoprotection glycerol was added to 25% v/v, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 294K |