4DJC
1.35 A crystal structure of the NaV1.5 DIII-IV-Ca/CaM complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-08 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9749 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.380, 72.720, 97.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.550 - 1.350 |
| R-factor | 0.15391 |
| Rwork | 0.153 |
| R-free | 0.17626 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.620 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.380 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 44657 | |
| Completeness [%] | 99.0 | 92.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M MES, 60% Isopropanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
