4DIB
The crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Bacillus anthracis str. Sterne
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.347, 102.044, 102.144 |
| Unit cell angles | 90.32, 73.99, 68.99 |
Refinement procedure
| Resolution | 40.000 - 2.550 |
| R-factor | 0.213 |
| Rwork | 0.208 |
| R-free | 0.26960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cmc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.492 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.060 | |
| Number of reflections | 87718 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 97.2 | 89.7 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M lithium sulfate, 25.5% PEG3350, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
