4DHY
Crystal structure of human glucokinase in complex with glucose and activator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-05 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.010, 82.200, 86.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.900 - 2.380 |
| R-factor | 0.1858 |
| Rwork | 0.183 |
| R-free | 0.24420 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3f9m |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.900 | 2.510 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.109 | 0.403 |
| Number of reflections | 18951 | |
| <I/σ(I)> | 12.5 | 3.1 |
| Completeness [%] | 96.8 | 92.5 |
| Redundancy | 5.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
