4DG0

Crystal structure of myristoylated WT catalytic subunit of cAMP-dependent protein kinase in complex with SP20 and AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-13
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	57.800, 78.730, 99.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.370 - 2.000
R-factor	0.196
Rwork	0.194
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.951
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.370	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	31277
<I/σ(I)>		4.2
Completeness [%]	100.0	100
Redundancy		6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	277.15	Mother liquor: 10% MPD Protein solution: 50 mM Bicine, 150 mM Ammonium Acetate, 8-10 mg/mL 9% MeOH added to the well immediately before sealing. 8 uL drop size with 1:1 protein:mother liquor, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K, pH 8.0