4DFM
Crystal structure of the large fragment of DNA polymerase I from Thermus aquaticus in ternary complex with 5-(aminopentinyl)-2-dCTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.99987 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 108.260, 108.260, 90.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.878 - 1.886 |
R-factor | 0.1875 |
Rwork | 0.186 |
R-free | 0.21620 |
Structure solution method | Difference Fourier Techniques |
Starting model (for MR) | 3m8s |
RMSD bond length | 0.008 |
RMSD bond angle | 0.992 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.7.3_928) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.150 | 1.940 |
High resolution limit [Å] | 1.886 | 1.890 |
Number of reflections | 48761 | |
<I/σ(I)> | 12.77 | 1.83 |
Completeness [%] | 98.6 | 82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 18% PEG 8000, 200mM Mg-Formate, 100 mM Tris pH 8.0 , VAPOR DIFFUSION, HANGING DROP, temperature 291K |