4DEY

Crystal structure of the Voltage Dependent Calcium Channel beta-2 Subunit in Complex With The CaV1.2 I-II Linker.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.24
Spacegroup name	P 21 21 21
Unit cell lengths	53.257, 70.512, 134.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.398 - 1.950
R-factor	0.2006
Rwork	0.199
R-free	0.22910
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	2.800	2.020
High resolution limit [Å]	1.950	2.700	1.950
Rmerge		0.334	0.338
Number of reflections	37774
<I/σ(I)>		3.3	1.2
Completeness [%]	91.0	99.2	36.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	292	1.1-1.25 M potassium sodium tartrate, 0.1 M Tris Soaked with KI before freezing , pH 7.5, VAPOR DIFFUSION, temperature 292K
2	VAPOR DIFFUSION	7.5	292	1.1-1.25 M potassium sodium tartrate, 0.1 M Tris Soaked with KI before freezing, pH 7.5, VAPOR DIFFUSION, temperature 292K
3	VAPOR DIFFUSION	7.5	292	1.1-1.25 M potassium sodium tartrate, 0.1 M Tris Soaked with NaBr before freezing, pH 7.5, VAPOR DIFFUSION, temperature 292K