4DEU
Crystal Structure of the Wild Type TTR Binding Naringenin (TTRwt:NAR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.46 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.734, 85.173, 65.058 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.254 - 1.599 |
| R-factor | 0.1906 |
| Rwork | 0.189 |
| R-free | 0.21330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.045 |
| RMSD bond angle | 2.088 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.599 |
| Rmerge | 0.064 |
| Number of reflections | 29385 |
| <I/σ(I)> | 11.1 |
| Completeness [%] | 87.0 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M calcium chloride, 0.1 M HEPES, pH 7.5, 28% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
