4DEQ

Structure of the Neuropilin-1/VEGF-A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-21
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 43
Unit cell lengths	114.978, 114.978, 50.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 2.649
R-factor	0.21155
Rwork	0.209
R-free	0.26698
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qqi
RMSD bond length	0.005
RMSD bond angle	1.011
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.649
Number of reflections	18014
Completeness [%]	91.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.5M Na/K phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K