4DE4
Crystal structure of aminoglycoside phosphotransferase APH(2")-Id/APH(2")-IVa in complex with HEPES
Replaces: 3R80Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97940 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.541, 101.214, 71.166 |
Unit cell angles | 90.00, 97.51, 90.00 |
Refinement procedure
Resolution | 28.988 - 2.000 |
R-factor | 0.2243 |
Rwork | 0.222 |
R-free | 0.27220 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.599 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 39540 | |
<I/σ(I)> | 35.71 | 3.11 |
Completeness [%] | 97.9 | 97.3 |
Redundancy | 3.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M NASCN, 20% PEG3350, 2 MM GTP, 2.5 MM GENTAMICIN, PH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |