4DBB

The PTB domain of Mint1 is autoinhibited by a helix in the C-terminal linker region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-05-10
Detector	R-AXIS IV
Spacegroup name	P 41 21 2
Unit cell lengths	64.729, 64.729, 115.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.016 - 1.901
R-factor	0.198
Rwork	0.197
R-free	0.22520
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.443
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.920
High resolution limit [Å]	1.900	6.490	1.900
Rmerge	0.066	0.020
Number of reflections	19927
<I/σ(I)>	11.6
Completeness [%]	99.5	99.2	97.9
Redundancy	13.6	11.9	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% isopropanol, 25% glycerol, 0.1 M Hepes pH 7.5, 0.15 M NaCl, 0.2 M ammonium acetate, 2 mM TCEP, vapor diffusion, hanging drop, temperature 293K