4DBB
The PTB domain of Mint1 is autoinhibited by a helix in the C-terminal linker region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-05-10 |
Detector | R-AXIS IV |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 64.729, 64.729, 115.155 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.016 - 1.901 |
R-factor | 0.198 |
Rwork | 0.197 |
R-free | 0.22520 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.443 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
High resolution limit [Å] | 1.900 | 6.490 | 1.900 |
Rmerge | 0.066 | 0.020 | |
Number of reflections | 19927 | ||
<I/σ(I)> | 11.6 | ||
Completeness [%] | 99.5 | 99.2 | 97.9 |
Redundancy | 13.6 | 11.9 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 25% isopropanol, 25% glycerol, 0.1 M Hepes pH 7.5, 0.15 M NaCl, 0.2 M ammonium acetate, 2 mM TCEP, vapor diffusion, hanging drop, temperature 293K |