4D9O
Structure of ebolavirus protein VP24 from Reston
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.438, 103.890, 59.773 |
Unit cell angles | 90.00, 93.94, 90.00 |
Refinement procedure
Resolution | 31.300 - 2.000 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.22670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.047 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 31125 | |
<I/σ(I)> | 21 | |
Completeness [%] | 98.7 | 98.7 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 295 | 0.1M Bis-Tris, 0.2M NaCl, 8% PEG3350, 14%(w/v) D-(+)-sucrose, pH 5.5, VAPOR DIFFUSION, temperature 295K |