4D61
Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | TVIPS TEMCAM-F416 (4k x 4k) |
| Number of Images | 1 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 9 |
| Software | UCSF Chimera,SPARX,SPIDER |
| Symmetry | C1 |
