4D52
CRYSTAL STRUCTURE OF FUCOSE BINDING LECTIN FROM ASPERGILLUS FUMIGATUS (AFL) IN COMPLEX WITH L-GALACTOPYRANOSE.
Replaces: 4UQ7Replaces: 4AHBExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.030, 70.440, 117.800 |
| Unit cell angles | 90.00, 108.34, 90.00 |
Refinement procedure
| Resolution | 111.820 - 1.760 |
| R-factor | 0.15493 |
| Rwork | 0.154 |
| R-free | 0.17780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4agi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.455 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.440 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 0.390 |
| Number of reflections | 121067 | |
| <I/σ(I)> | 12 | 3.2 |
| Completeness [%] | 97.1 | 96.1 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM CACL2, 25% PEG 4K AND 100 MM TRIS, PH 8.5 |
