4D4S
Focal Adhesion Kinase catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.266, 124.877, 50.814 |
| Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
| Resolution | 62.440 - 2.000 |
| R-factor | 0.20731 |
| Rwork | 0.206 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jkk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.049 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.440 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.360 |
| Number of reflections | 37958 | |
| <I/σ(I)> | 12.7 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS PH 8.5, 0.075M LISO4, 20% PEG4000, 10 MM TCEP |
