4D3X
The structure of mature legumain from chinese hamster.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 |
| Unit cell lengths | 43.620, 43.620, 209.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.776 - 1.848 |
| R-factor | 0.1767 |
| Rwork | 0.174 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4awa |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.061 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.050 | 0.010 |
| Number of reflections | 33931 | |
| <I/σ(I)> | 25.8 | 4.7 |
| Completeness [%] | 88.7 | 19.2 |
| Redundancy | 6.1 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M NACL, 23%(W/V) PEG 3350, 0.1 M MOPS PH 6.5; THEN 10%(V/V) GLYCEROL WERE ADDED FOR CRYO-PROTECTION |
