4D2I
Crystal structure of the HerA hexameric DNA translocase from Sulfolobus solfataricus bound to AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2012-05-26 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | H 3 |
| Unit cell lengths | 150.210, 150.210, 140.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.733 - 2.841 |
| R-factor | 0.2133 |
| Rwork | 0.211 |
| R-free | 0.25410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.706 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.730 | 2.940 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 26776 | |
| <I/σ(I)> | 13.06 | 2.72 |
| Completeness [%] | 96.0 | 94.4 |
| Redundancy | 2.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | PROTEIN WAS CRYSTALLIZED FROM 20% V/V GLYCEROL ETHOXYLATE, 12.5% V/V TETRAHYDROFURAN, 100 MM TRIS-HCL PH 8 |
