4D2E
Crystal structure of an integral membrane kinase - v2.3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-21 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.290, 91.820, 143.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.021 - 2.280 |
R-factor | 0.1904 |
Rwork | 0.188 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ze3 |
RMSD bond length | 0.012 |
RMSD bond angle | 0.995 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.290 | 2.340 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.090 | 0.810 |
Number of reflections | 45427 | |
<I/σ(I)> | 15.9 | 1.9 |
Completeness [%] | 98.4 | 96.9 |
Redundancy | 9.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 277 | 3-5%(V/V) 2-METHYL-2, 4-PENTANEDIOL (MPD), 0.1 M SODIUM CHLORIDE, 0.06 M MAGNESIUM ACETATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPID CUBIC PHASE) METHOD WITH 7.8 MAG AS THE HOST LIPID AT 4 CELSIUS DEGREE. |