4D1U

A D120A mutant of VIM-7 from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 43
Unit cell lengths	69.764, 69.764, 47.286
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.665 - 1.800
R-factor	0.163
Rwork	0.161
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y8b
RMSD bond length	0.012
RMSD bond angle	1.298
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.080	0.540
Number of reflections	21271
<I/σ(I)>	13.2	2.6
Completeness [%]	100.0	100
Redundancy	4.2	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1