4D1J
The structure of the GH35 beta-galactosidase Bgl35A from Cellvibrio japonicas in complex with 1-Deoxygalactonojirimycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-26 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 99.257, 116.095, 116.113 |
Unit cell angles | 90.00, 90.05, 90.04 |
Refinement procedure
Resolution | 116.110 - 1.800 |
R-factor | 0.17286 |
Rwork | 0.172 |
R-free | 0.19482 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u7v |
RMSD bond length | 0.011 |
RMSD bond angle | 1.466 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | 0.570 |
Number of reflections | 466658 | |
<I/σ(I)> | 9.8 | 1.4 |
Completeness [%] | 97.1 | 95.8 |
Redundancy | 2.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 2.8 M SODIUM ACETATE PH 7.0 |