4D0E
Human Notch1 EGF domains 11-13 mutant GlcNAc-fucose disaccharide modified at T466
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 28.440, 28.440, 282.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.070 - 1.610 |
| R-factor | 0.23238 |
| Rwork | 0.231 |
| R-free | 0.27031 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vj3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.103 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.070 | 1.650 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.040 | 0.410 |
| Number of reflections | 16974 | |
| <I/σ(I)> | 15.3 | 2.3 |
| Completeness [%] | 96.6 | 92.9 |
| Redundancy | 4.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6.0 |
