4D05
Structure and activity of a minimal-type ATP-dependent DNA ligase from a psychrotolerant bacterium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 178.427, 43.976, 89.633 |
Unit cell angles | 90.00, 105.92, 90.00 |
Refinement procedure
Resolution | 24.610 - 1.650 |
R-factor | 0.1468 |
Rwork | 0.145 |
R-free | 0.19240 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.013 |
RMSD bond angle | 1.439 |
Data reduction software | XDS |
Phasing software | SHELX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.070 | 0.600 |
Number of reflections | 80785 | |
<I/σ(I)> | 13.3 | 2.1 |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 4.5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.75 M AMMONIUM SULFATE, 100 MM HEPES PH 7.4 |