4CZM
C. crescentus MreB, monomeric, AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.791, 67.791, 320.556 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.138 - 2.200 |
R-factor | 0.212 |
Rwork | 0.209 |
R-free | 0.27660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jce |
RMSD bond length | 0.018 |
RMSD bond angle | 1.893 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.060 | 1.500 |
Number of reflections | 39419 | |
<I/σ(I)> | 19.5 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.9 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |