4CZH
C. crescentus MreB, single filament, ADP, MP265 inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.618, 73.739, 53.700 |
| Unit cell angles | 90.00, 102.38, 90.00 |
Refinement procedure
| Resolution | 26.226 - 1.644 |
| R-factor | 0.2021 |
| Rwork | 0.201 |
| R-free | 0.23230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jce |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.670 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.060 | 0.620 |
| Number of reflections | 45534 | |
| <I/σ(I)> | 14 | 2.2 |
| Completeness [%] | 98.4 | 95 |
| Redundancy | 6.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
