4CZF

C. crescentus MreB, single filament, ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	51.548, 71.815, 53.731
Unit cell angles	90.00, 100.37, 90.00

Refinement procedure

Resolution	21.406 - 1.640
R-factor	0.1736
Rwork	0.172
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jce
RMSD bond length	0.017
RMSD bond angle	1.630
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.640	1.640
Rmerge	0.030	0.150
Number of reflections	46866
<I/σ(I)>	42.1	10.7
Completeness [%]	99.3	99.3
Redundancy	7.1	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1