4CZE
C. crescentus MreB, double filament, empty
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 52.270, 98.150, 90.289 |
| Unit cell angles | 90.00, 98.10, 90.00 |
Refinement procedure
| Resolution | 34.241 - 2.000 |
| R-factor | 0.1984 |
| Rwork | 0.197 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jce |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.026 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.380 |
| Number of reflections | 29232 | |
| <I/σ(I)> | 7 | 3.3 |
| Completeness [%] | 96.1 | 98.3 |
| Redundancy | 3.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
