4CY2
Crystal structure of the KANSL1-WDR5-KANSL2 complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.590, 86.600, 45.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.300 - 2.000 |
| R-factor | 0.20057 |
| Rwork | 0.199 |
| R-free | 0.22183 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g99 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.041 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.150 | 0.840 |
| Number of reflections | 22261 | |
| <I/σ(I)> | 10.7 | 2.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 20% (W/V) PEG 3350, 0.2 M POTASSIUM NITRATE, PH 6.9. |
