4CY1
Crystal structure of the KANSL1-WDR5 complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-29 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.760, 92.450, 81.170 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 40.580 - 1.500 |
| R-factor | 0.17057 |
| Rwork | 0.170 |
| R-free | 0.18344 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g99 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.007 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.640 |
| Number of reflections | 90649 | |
| <I/σ(I)> | 13.6 | 2.4 |
| Completeness [%] | 96.7 | 89 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 26% (W/V) PEG 3350, 0.2 M AMMONIUM SULPHATE, 0.1 M BIS-TRIS, PH 6.5. |
