4CXL
Human insulin analogue (D-ProB8)-insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-16 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 78.660, 78.660, 78.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.330 - 1.500 |
| R-factor | 0.1683 |
| Rwork | 0.167 |
| R-free | 0.19713 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mso |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.784 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.330 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.660 |
| Number of reflections | 13097 | |
| <I/σ(I)> | 31.8 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M TRIS/HCL PH 8.0, 0.2 SODIUM CITRATE, 40% V/V MPD, PROTEIN CONCENTRATION 5MG/ML IN 20 MM HCL |
