4CXG
Regulation of the mammalian elongation cycle by 40S subunit rolling: a eukaryotic-specific ribosome rearrangement
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 230 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8.7 |
| Software | Coot,UCSF Chimera,SPARX,SPIDER |
| Symmetry | C1 |
