4CWC
Structure of Rolling Circle Replication Initiator Protein (RepDE) from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-08 |
| Detector | ADSC Q4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 240.300, 56.536, 62.357 |
| Unit cell angles | 90.00, 102.22, 90.00 |
Refinement procedure
| Resolution | 49.990 - 2.900 |
| R-factor | 0.21858 |
| Rwork | 0.216 |
| R-free | 0.25894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cij |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.504 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.940 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.130 | 0.590 |
| Number of reflections | 17447 | |
| <I/σ(I)> | 9.3 | 1.5 |
| Completeness [%] | 96.4 | 100 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.5 M AMMONIUM CITRATE/AMMONIUM HYDROXIDE PH 8.5, 15% (W/V) PEG 8000 |
