4CVZ

COMPLEX OF A B21 CHICKEN MHC CLASS I MOLECULE AND A 10MER CHICKEN PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Collection date	2013-04-20
Spacegroup name	P 21 21 21
Unit cell lengths	60.520, 69.030, 93.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.840 - 2.390
R-factor	0.2586
Rwork	0.258
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2YF6
RMSD bond length	0.007
RMSD bond angle	0.850
Data reduction software	XDS (THROUGH XIA2)
Data scaling software	Aimless (THROUGH XIA2)
Phasing software	PHASER
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.840	2.450
High resolution limit [Å]	2.390	2.390
Rmerge	0.150	0.910
Number of reflections	16087
<I/σ(I)>	11.9	2.1
Completeness [%]	99.9	100
Redundancy	6.4	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		0.1M SODIUM ACETATE, PH 5.0, 1.5M AMMONIUM SULFATE.