4CU8
Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 116.550, 116.550, 219.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 116.550 - 2.500 |
R-factor | 0.16374 |
Rwork | 0.161 |
R-free | 0.21147 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.427 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.130 | 0.440 |
Number of reflections | 53140 | |
<I/σ(I)> | 16.2 | 6.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.5 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |