4CT0
Crystal Structure of Mouse Cryptochrome1 in Complex with Period2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-03 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 99.736, 99.736, 178.632 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.496 - 2.450 |
| R-factor | 0.1884 |
| Rwork | 0.186 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k0r |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 45.500 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.060 | 1.050 |
| Number of reflections | 33988 | |
| <I/σ(I)> | 35 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.1 | 26.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MGCL2, 12-13% PEG3350 |
