4CSY
E-selectin lectin, EGF-like and two SCR domains complexed with Sialyl Lewis X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.290, 58.670, 58.870 |
| Unit cell angles | 76.03, 86.28, 86.31 |
Refinement procedure
| Resolution | 28.320 - 2.410 |
| R-factor | 0.2168 |
| Rwork | 0.215 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1G1S 3gov 1h04 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.280 | 2.530 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.040 | 0.320 |
| Number of reflections | 25239 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 95.4 | 90.2 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 13 % PEG8000, HEPES, MOPS PH 6.2, CACL2, 10MM SLEX-OTMSE |
