4CSA
Crystal structure of the asymmetric human metapneumovirus M2-1 tetramer bound to a DNA 4-mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.140, 93.900, 85.510 |
| Unit cell angles | 90.00, 95.82, 90.00 |
Refinement procedure
| Resolution | 31.670 - 2.280 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.22210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cs8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.040 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.050 | 0.840 |
| Number of reflections | 35938 | |
| <I/σ(I)> | 19.6 | 1.8 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 6.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 28 % W/V POLYETHYLENE GLYCOL MONOMETHYL ETHER 2000, 0.100 M BIS-TRIS PH 6.5 |
