4CPH
trans-divalent streptavidin with love-hate ligand 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-28 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.740, 47.490, 104.880 |
| Unit cell angles | 90.00, 101.14, 90.00 |
Refinement procedure
| Resolution | 41.902 - 1.640 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ry1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.229 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.080 | 0.590 |
| Number of reflections | 52123 | |
| <I/σ(I)> | 8.4 | 1.9 |
| Completeness [%] | 96.6 | 95.5 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 75% AMMONIUM SULPHATE, 25% SODIUM ACETATE PH4.5. SITTING DROP |
