4CPC
Crystal structure of human synaptonemal complex protein SYCP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.140, 92.381, 103.367 |
| Unit cell angles | 66.53, 82.32, 76.53 |
Refinement procedure
| Resolution | 47.360 - 2.240 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAD SOLUTION OF THE IODIDE DERVIATIVE STRUCTURE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.360 | 2.290 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.150 | 1.090 |
| Number of reflections | 66412 | |
| <I/σ(I)> | 11 | 2.5 |
| Completeness [%] | 86.7 | 38.9 |
| Redundancy | 9.7 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN WAS CRYSTALLISED FROM 100 MM HEPES PH 7.5, 100 MM NACL, 13.0% (W/V) PEG3350; THEN SOAKED IN 20% GLYCEROL. |
