4CNT
CRYSTAL STRUCTURE OF WT HUMAN CRMP-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.370, 157.650, 86.380 |
| Unit cell angles | 90.00, 113.03, 90.00 |
Refinement procedure
| Resolution | 55.970 - 2.650 |
| R-factor | 0.18913 |
| Rwork | 0.187 |
| R-free | 0.23563 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cns |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.636 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.790 | 2.720 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.190 | 1.330 |
| Number of reflections | 61101 | |
| <I/σ(I)> | 5.5 | 1 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 15% (W/V) PEG 6000, 100 MM NAHEPES PH 7.5, 100 MM KCL |
