4CNS
Crystal structure of truncated human CRMP-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.400, 89.620, 133.100 |
| Unit cell angles | 90.00, 101.40, 90.00 |
Refinement procedure
| Resolution | 30.990 - 2.400 |
| R-factor | 0.19092 |
| Rwork | 0.189 |
| R-free | 0.22581 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b91 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.507 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.210 | 2.450 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.170 | 0.540 |
| Number of reflections | 75030 | |
| <I/σ(I)> | 4.8 | 1.4 |
| Completeness [%] | 96.5 | 93.5 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 30% (W/V) PEG 3350, 100 MM NA TARTRATE, 100 MM BIS-TRIS PROPANE 6.5 |
