4CNS
Crystal structure of truncated human CRMP-4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-25 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.400, 89.620, 133.100 |
Unit cell angles | 90.00, 101.40, 90.00 |
Refinement procedure
Resolution | 30.990 - 2.400 |
R-factor | 0.19092 |
Rwork | 0.189 |
R-free | 0.22581 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b91 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.507 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.210 | 2.450 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.170 | 0.540 |
Number of reflections | 75030 | |
<I/σ(I)> | 4.8 | 1.4 |
Completeness [%] | 96.5 | 93.5 |
Redundancy | 2.5 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 30% (W/V) PEG 3350, 100 MM NA TARTRATE, 100 MM BIS-TRIS PROPANE 6.5 |